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mRNA Fate and Dynamics Following microRNA-Direcged Translational Repression
Joseph D. Shih, Zeev Waks, Pamela A. Silver. Harvard Medical School

A synthetic miRNA-regulated cell line was created to track the fate of miRNA-regulated mRNA containing an exogenous luciferase mRNA as a reporter gene. In order to determine the dynamics of proteins complexing with miRNA-regulated mRNA, single molecule in situ hybridization of the target mRNA was conducted in combination with immunofluorescence to identify factors involved in degradation, storage, and reactivation of miRNA-regulated mRNA.

Digital Volumetric Imaging of Angiogenesis in Tissue Engineered Constructs
Marc M. Takeno, Kip D. Hauch. University of Washington

Accurate identification of functional vasculature is of critical importance in evaluating construct integration. Digital volumetric imaging (DVI) is an automated serial sectioning and microscopic imaging technique that can bridge the resolution gap between micro-computed tomography and conventional histology. We use DVI to study in vivo vascularization of tissue engineering constructs in a variety of settings.

Optical Imaging of Cerebellar Dysfunction in SCA1 Mice
Nathan Jorgensen, Gang Chen, Wangcai Gao, Timothy Ebner, Harry Orr. University of Minnesota

Spinocerebellar ataxia type 1(SCA1) is an inherited cerebellar neurodegenerative disorder caused by a polyglutamine tract expansion in the ataxin-1 protein.

Training rural Guatemalan midwives to assess newborn gestational age
KC Bly, Christina Ha, Teresa Keirns, Amy Levi, Lisa Thompson. UCSF

Half of rural Guatemala's population is indigenous; most births are attended at home by traditional midwives. Perinatal mortality is 2nd highest in Latin America. We administered a survey and implemented a training aimed at reducing perinatal mortality.

Chemically Modified Primers for Improved Multiplexed PCR
Elena Hidalgo Ashrafi, Tony Le, Alexandre Lebedev, Richard Hogrefe, Victor Timoshchuk, Sabrina Shore, Inna Koukhareva and Natasha Paul. TriLink BioTechnologies

Multiplex PCR is an advantageous technique used in PCR applications to amplify multiple targets in a single reaction. As useful as it is, this technique presents a new set of challenges that further complicates PCR setup. For example, reactions are more prone to off-target amplifications such as mis-priming and primer dimer due to the increased number of primer pairs. Furthermore, preferential amplification of certain targets leads to an unequal distribution of amplicon products, making quantifi

Hot Start dNTPs - A Novel Tool for Controlled Nucleotide Incorporation in PCR
Tony Le, Elena Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev. TriLink BioTechnologies

PCR is a widely used scientific tool employed by a variety of applications. Various Hot Start technologies have already been developed using modified PCR components to increase specificity of a reaction. Recently developed CleanAmpTM dNTPs are modified nucleoside triphosphates with a thermolabile 3’-tetrahydrofuranyl protecting group that is released at higher temperatures. These modified dNTPs prevent low temperature primer extension, which can often be a significant problem in PCR. At higher t

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