Real-time Microarrays – The Path to Quantitation?

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Launch presentation

About the speaker

Steve Blair received the B.S. and M.S. degrees in Applied Optics from Rose-Hulman Institute of Technology, Terre Haute, IN, in 1991 and 1993, respectively, and the Ph.D. degree in Electrical Engineering from the University of Colorado, Boulder, in 1998. Since 1998, he has been with the Electrical and Computer Engineering Department, University of Utah, Salt Lake City, and is now an Associate Professor. His research interests include nonlinear optics, plasmonics, and microarray technology and applications.

Abstract

The current state of microarray technology allows qualitative answers at best, due, in part, to the complexity of cross-hybridization and the effects of incomplete reactions. Real-time microarray technology monitors the reactions across the array as they progress, providing significantly more data. Extracting quantitative information from this data requires kinetics-based analysis methods, and new assay methodologies can be developed which fully utilize this overall approach. This talk will overview real-time instrumentation and kinetic analysis and will compare this approach to microarray experimentation to that of current platforms. Limits to quantitative data interpretation will be discussed, in particular, in the context of heteroplasmy detection in mtDNA.

Launch presentation